Figure 5.

Citrate transport (pH 5.5, 30 °C) in S. cerevisiae IMX1000 cells expressing C. jadinii transporters. (A) Uptake of ¹⁴C-citric acid 5 mM in cells expressing the transporters CjJEN6, CjSLC16, CjSLC5, CjTDT, CjSLC13-1, CjSLC13-2, and CjATO5, as well as those transformed with empty vector (pɸ). Statistical significance was estimated using one-way ANOVA followed by a post hoc Tukey’s multiple comparison test as follows: * p < 0.1 and **** p < 0.0001, citrate uptake significantly different from cells transformed with the empty plasmid. (B) Initial uptake rates of radiolabeled ¹⁴C-citric acid as a function of the acid concentration in cells expressing CjJen6, CjSlc5, and CjAto5; the table summarizes the kinetic parameters estimated with the computer-assisted non-linear regression analysis of the plots.