Table 2.

Summary of amino acid mutations in spike relevant for VOC identification

	69/70	417	452	484	501	681	371/373
Omicron	del	N	L	A	Y*	H#	L/P
Alpha	del	K	L	E	Y	H	S/S
Beta	H/V	N	L	K	Y	P	S/S
Gamma	H/V	T	L	K	Y	P	S/S
Delta	H/V	K	R	E	N	R	S/S

Amino acid positions in the SARS-CoV-2 spike protein critical for the identification of VOCs alpha, beta, gamma, delta and omicron are depicted

*Melting curve peak not detected in assay A

^#Peak shifted due to surrounding mutations