Figure 1. LPS or TNF-α induced IL-1β and IL-18 secretion in by Nlrp3^CA/+;Gsdmd^−/− but not Gsdmd^−/− mice.

Three-month-old WT, Gsdmd^−/−, Nlrp3^CA/+, and Nlrp3^CA/+;Gsdmd^−/− mice were injected with 15 mg/kg LPS for 6 hours or 0.5 mg/kg TNF-α for 2 hours. PBS-administrated mice served as controls. N=4-6 mice/group. Serum cytokine levels were measured by V-PLEX Plus Proinflammatory Panel 1 Mouse Kit, except for IL-18, which were assessed by ELISA. Data are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. One-Way ANOVA. LPS, lipopolysaccharide; IL, interleukin; WT, wild type; ca, constitutive activation; TNF-α, tumor necrosis factor α.