Table 2.
Amino acid sequence comparison of MW627239 (goshawk, Italy, 2020) and representative WNV-L1 Italian, European, and American strains, conducted using BioEdit v. 7.2 software. The strains (i) 115803 (accession number: FJ483548, Italy, 2008); (ii) WNV Italy 1998-equine (accession number: AF404757, Italy, 1998); (iii) Italy/2008/M-203204 (accession number: JF719066, Italy, 2008); (iv) Italy/2008/J-242853 (accession number: JF719065, Italy, 2008); (v) 21412 (accession number: MW835362, Italy, 2011); (vi) Italy/2013/Livenza/35.1 (accession number: KF647253, Italy, 2013); (vii) Spain/2010/H-1b (accession number: JF719069, Spain, 2010); (viii) GE-2o/V (accession number: FJ766332, Spain, 2007); (ix) Akela/France/2015 (accession number: MT863559, France, 2015); and (x) NY99 (accession number: NC009942, USA, 1999) were included in the analysis. Only representative amino acid residues were analyzed. C protein: amino acid (aa) 1–105; PreM protein: aa 124–290; M protein: aa 216–290; E protein: aa 291–791; NS1 protein: aa 792–1143; NS2A protein: 1114–1374; NS2B protein: 1375–1505; NS3 protein: 1506–2124; NS4A protein: 2125–2250; NS4B protein: 2274–2528; NS5 protein: 2529–3433. Significant amino acid residue substitutions are highlighted in red.
| Viral Protein | Amino Acid Position |
MW627239 ITA 2020 | AF404757 ITA 1998 | JF719066 ITA 2008 | FJ766332 SPA 2007 | JF719065 ITA 2008 | FJ483548 ITA 2008 | MW835362 ITA 2011 | KF647253 ITA 2013 | NC_009942 USA 1999 | JF719069 SPA 2010 | MT863559 FRA 2015 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C | 34 | M | M | M | V | M | M | M | M | M | M | M |
| 100 | S | S | S | S | S | S | S | S | S | S | S | |
| prM | 72 | S | S | S | S | S | S | S | S | S | S | S |
| M | 36 | I | I | I | I | I | I | I | I | I | I | I |
| E | 35 | S | S | S | S | S | S | S | S | S | S | S |
| 51 | T | A | A | T | A | A | A | A | A | A | A | |
| 76 | T | T | T | T | T | T | T | T | T | T | T | |
| 88 | P | P | P | S | P | P | P | P | P | P | P | |
| 126 | T | T | T | T | T | T | T | T | I | T | T | |
| 153 | G | G | G | G | G | G | G | G | G | G | G | |
| 159 | I | I | I | I | I | I | I | I | V | I | I | |
| 278 | T | T | T | T | T | T | T | T | T | T | T | |
| 312 | L | L | L | L | L | L | L | L | L | L | L | |
| 442 | V | V | V | V | V | V | V | V | V | V | V | |
| NS1 | 17 | S | S | S | S | S | S | S | S | S | S | S |
| 35 | Y | Y | Y | H | Y | Y | Y | Y | Y | Y | Y | |
| 45 | I | I | I | I | I | I | I | I | I | I | I | |
| 70 | S | S | S | S | S | S | S | S | A | S | S | |
| 94 | E | E | E | E | E | E | E | E | E | E | E | |
| 138 | P | P | P | P | P | P | P | P | P | P | P | |
| 141 | K | K | K | K | K | K | K | K | K | K | K | |
| 188 | V | V | V | V | V | V | V | V | V | V | V | |
| 208 | D | D | D | H | D | D | D | D | D | D | D | |
| 288 | S | S | S | S | S | S | S | S | S | S | S | |
| 289 | E | E | E | G | E | E | E | E | E | E | E | |
| NS2A | 85 | I | I | V | I | V | V | I | I | I | I | I |
| 104 | N | N | N | N | N | N | N | N | N | N | N | |
| 119 | H | H | H | H | H | H | H | H | H | H | H | |
| 128 | E | E | E | E | E | E | E | E | E | E | E | |
| 138 | V | V | V | V | V | V | V | V | V | V | V | |
| 165 | G | G | G | G | G | G | G | G | G | G | G | |
| NS2B | 82 | D | D | D | D | D | D | D | D | D | D | D |
| 83 | G | G | G | G | G | G | G | G | G | G | G | |
| 103 | A | A | A | A | A | A | A | V | V | A | A | |
| 120 | V | I | V | V | V | I | V | V | V | V | V | |
| NS3 | 46 | F | F | F | F | F | F | F | F | F | F | F |
| 244 | Q | Q | Q | Q | Q | Q | Q | Q | Q | Q | H | |
| 249 | T | T | T | P | T | P | T | P | P | T | T | |
| 356 | I | I | I | I | I | I | I | I | T | I | I | |
| 496 | L | L | L | L | L | L | L | L | L | L | L | |
| 503 | N | N | N | N | N | N | N | N | N | N | N | |
| 521 | D | D | D | D | D | D | D | D | D | D | D | |
| NS4A | 85 | V | V | I | V | I | I | V | V | A | V | V |
| 100 | P | P | S | P | S | S | P | P | P | P | P | |
| 122 | P | P | P | P | P | P | P | P | P | P | P | |
| NS5 | 53 | H | H | H | H | H | H | H | H | H | H | H |
| 54 | P | P | P | P | P | P | P | P | P | P | P | |
| 257 | D | D | D | D | D | D | D | D | D | D | D | |
| 258 | V | V | A | V | A | A | A | V | V | V | V | |
| 280 | K | K | K | K | K | K | K | K | K | K | K | |
| 372 | V | V | V | V | V | V | V | V | V | V | V | |
| 374 | Y | Y | Y | Y | Y | Y | Y | Y | Y | Y | Y | |
| 422 | R | R | K | R | K | K | K | R | R | R | R | |
| 426 | E | E | E | A | E | E | E | E | E | E | E | |
| 436 | M | M | M | I | M | M | M | M | M | M | M | |
| 526 | T | T | T | T | T | T | T | T | T | T | T | |
| 653 | F | F | F | F | F | F | F | F | F | F | F | |
| 681 | T | T | T | T | T | T | T | T | T | T | T |