Figure 3.

Effects of hkMJ2 on the expression levels of osteoclast differentiation-related genes and proteins in the RANKL-induced cells. The expression levels of RANKL-induced osteoclastogenic genes were measured by qPCR (A) and proteins were measured by western blotting (B) and quantified (C). The expression levels of NFATc1-downstream genes were measured by qPCR (D). The values indicate the mean ± standard deviation (SD) of three independent experiments performed in triplicate. NFATc1, nuclear factor of activated T-cells cytoplasmic 1; RANKL, receptor activator of nuclear factor-κB ligand; RANK, receptor activator of nuclear factor-κB; NF-κB, nuclear factor kappa light chain enhancer of activated B cells; Atp6v0d2, V-type proton ATPase subunit d2; Calcr, calcitonin receptor; Ctsk, cathepsin K. −, no addition; +, addition. One way ANOVA followed by Tukey’s HSD was used to determine significance.