Figure 7.

Inhibitory effect of MJ2 on osteoclast differentiation in CIA mice (n = 8 per group). Osteoclasts were stained with TRAP staining in mice knee joints (100×, scale bar = 100 μm) (A), and TRAP(+) cells were counted (B). Expression levels of osteoclast differentiation-related genes (C) and OPG/RANKL ratio (D) were measured by qPCR. Data values indicate the mean ± standard error of the mean (SEM). One-way ANOVA followed by Tukey’s HSD was used to determine significance. CIA, collagen-induced arthritis; TRAP, tartrate-resistant acid phosphatase; NFATc1, nuclear factor of activated T-cells cytoplasmic 1; Calcr, calcitonin receptor; Ctsk, cathepsin K; MMP-9, matrix metalloproteinase-9; RANKL, receptor activator of the nuclear factor-κB ligand; OPG, osteoprotegerin.