Figure 3.

Cryptocaryon irritans infection produces inflammation in gilthead seabream tissues. Transcription of inflammatory cytokines (a–c) in the gills and head kidney (HK) from the control (C) and C. irritans-infected (Ci) gilthead seabream after 2 days of a natural outbreak. Data represent the mean relative gene expression corrected by the ef1a expression in each sample ± SEM (n = 6) obtained by real-time PCR. (*) Asterisks indicate a significant difference between control and infected specimens according to Student’s t test (p ≤ 0.05).