Figure 5.

Natural cytotoxic cells, but not granzymes, are involved in the response against Cryptocaryon irritans. Transcription of genes related to the cell-mediated cytotoxicity (a–d) in the gills from control (C) or C. irritans-infected (Ci) gilthead seabream after 2 days of a natural outbreak. Data represent the mean relative gene expression corrected by the ef1a expression in each sample ± SEM (n = 6) obtained by real-time PCR. (*) Asterisks indicate a significant difference between control and infected specimens according to Student’s t test (p ≤ 0.05).