Figure 3.

Effect of NS5A-D2 expression on mitochondrial activity of Huh7-GCK⁺/HK2⁻ cells (A) Oxygen consumption rate (OCR) in Huh7-GCK⁺/HK2⁻ cells expressing or not NS5A-D2 was determined using the Mito Stress test on a Seahorse XFe24 analyzer before and after addition of oligomycin (Complex V inhibitor), FCCP (uncoupling agent), rotenone (Rot; Complex I inhibitor) and antimycin A (Anti-A; Complex III inhibitor). (B) Basal OCR, (C) ATP production, Maximal Respiration and Spare Respiration Capacity were calculated from (A). Data presented means ± SEM (n = 5) and p values were determined by Student’s t-test. (D) Energy map of Huh7-GCK⁺/HK2⁻ cells expressing or not NS5A-D2 determined from OCR and ECAR measured at basal stage in a Glycolytic Rate Test (Supplementary Figure S3), performed in XF assay medium with 5 mM or 25 mM glucose. Data presented means ± SEM (n = 12).