Figure 2.

Analysis of hACE2-RBD binding affinity by Biolayer Interferometry. (A–C) Binding kinetics between SARS-CoV-2 RBD (WT or different single mutant) assessed by BLI at two different temperatures. Biosensors coated with RBD proteins were incubated in two-fold dilution series of sACE2 (500 nM–31.25 nM) at 10 °C (A) and 25 °C (B) temperatures. Representative raw data are shown in blue and fitting model is shown in red. Graphs represent the affinity constants (K_D), on rates (K_a) and off rates (K_dis), (C) values obtained in two different experiments at two different temperatures and calculated using a 1:1 binding model. All BLI data are summarized in Table S1.