Figure 5.

Schematic demonstration of fundamental workflows for PacBio’s libraries’ construction. (a) The process describes the sequencing of targeted regions of genomic DNA without the need of amplification. The innovation lies in the usage of the CRISPR-Cas9 system for template enrichment. (b) Iso-seq™ SMRTbell^® workflow is designed to enable the characterization of full-length transcripts from total RNA. The procedure involves first-strand cDNA synthesis and PCR amplification, followed by the classic workflow for DNA sequencing library construction.