Table 3.
Presentation of the available PacBio’s and ONT’s sequencing approaches accompanied by their cost and additional information for the library preparation workflows. Of note, for the implementation of a sequencing run, the price of PacBio’s SMRT cell and ONT’s flow cell should be included in the overall cost.
| Platform | Approach | Average Cost | Preparation Time | Input Amount |
PCR Required |
|---|---|---|---|---|---|
| PacBio | SMRTbell Express for large DNA inserts |
~$730 | 4 h | 1–5 μg | No |
| SMRTbell Express for ultra-low DNA inputs |
~$850 | 5–8 h | 5–20 ng gDNA | Yes | |
| CRISPR-Cas 9 system | * | >10 h | 5 μg gDNA | No | |
| Iso-seq | ~$500 | 8 h | 300 ng total RNA | Yes | |
| 16S amplicon sequencing | ~$450 | PCR + 4 h | 25 pg–2.5 ng gDNA | Yes | |
| ONT | Rapid DNA sequencing | ~$200 | 10 min | 400 ng gDNA | No |
| DNA ligation sequencing | ~$350 | 60 min | 1000 ng dsDNA | No | |
| Cas9 Sequencing Kit | * | 110 min | 1–10 µg dsDNA | No | |
| PCR-cDNA sequencing | ~$430 | 165 min | 1 ng poly-A+ RNA (or 50 ng total RNA) |
Yes | |
| Direct cDNA sequencing | ~$400 | 275 min | 100 ng poly-A+ RNA | No | |
| Direct RNA sequencing | ~$350 | 105 min | 500 ng poly-A+ RNA | No | |
| 16S sequencing | ~$350 | PCR + 10 min | 10 ng gDNA | Yes |
* The average cost depends on the target.