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. 2022 Jan 10;12(1):55. doi: 10.3390/metabo12010055

Figure 4.

Figure 4

LNT improved oxidative stress and apoptosis in PA-induced AML12 cells. (A) DHE staining of AML-12 cells. (BE) MDA, GSH/GSSH, SOD, and 4-HNE levels in AML-12 cells. (F) Representative Western blots and quantification of SOD1, SOD2, NOX2, and NOX4 in AML-12 cells. (G) Representative annexin-V-FITC/PI staining and quantification of apoptosis by flow cytometry. (H) Cleaved-Caspase3 expression by Western blot analysis and Caspase3 activity in AML-12 cells. (I) Cell viability of AML-12 cells. (J) Representative Western blots and quantification for apoptosis-related proteins in AML-12 cells. Data are presented as the mean ± SEM (n = 3 per group). * p < 0.05 compared with the Control group. # p < 0.05 compared with the PA group.