Figure 1.

Comparison of the relative activities of rutaecarpine and NF-κB inhibitors in platelet aggregation and P-selectin expression in human platelets stimulated by collagen. Washed human platelets (3.6 × 10⁸ cells/mL) were preincubated with BAY11-7082, Ro106-9920, or rutaecarpine (RUT) at the same concentrations (1, 3, and 6 μM), followed by the addition of collagen (1 μg/mL) to trigger (A) platelet aggregation and (B) surface P-selectin expression (a, resting controla; b, collagen-activated; c, RUT 1 μM, BAY11-7082 1 μM or Ro106-9920 1 μM; d, RUT 3 μM, BAY11-7082 3 μM or Ro106-9920 3 μM; e, RUT 6 μM, BAY11-7082 6 μM or Ro106-9920 6 μM) as described in the Materials and Methods. The corresponding statistical data are displayed in the right (A) or below (B) panel of each figure. Data are presented as the mean ± standard error of the mean (n = 4). *** p < 0.001, compared with the 0.1% dimethyl sulfoxide (DMSO)-treated group (A) or resting group (Tyrode’s solution, (B)); ^## p < 0.01 and ^### p < 0.001, compared with the 0.1% DMSO-treated group (B).