Figure 4.

Confocal laser fluorescence of rutaecarpine, BAY11-7082, and SB203580 in the reduction in p65 phosphorylation in human platelets stimulated by collagen. Washed platelets (1.2 × 10⁹ cells/mL) were preincubated with rutaecarpine (RUT; 6 μM), BAY11-7082 (6 μM), or SB203580 (20 μM), followed by the addition of collagen (1 μg/mL) to trigger platelet aggregation. The confocal images of phosphorylated p65 (green fluorescence) and α-tubulin (blue fluorescence) were observed using goat anti-rabbit CF^TM 488A Dye and goat anti-mouse CF^TM 405M Dye, respectively. The profiles represent four similar studies. Bar: 2 μm.