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. 2022 Jan 12;27(2):476. doi: 10.3390/molecules27020476

Figure 5.

Figure 5

Inhibitory effect of rutaecarpine and BAY11-7082 on free radical formation in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and acute pulmonary thromboembolism as well as tail bleeding time in mice. (A) DPPH–methanol (400 μM) was mixed with rutaecarpine (RUT; 6 μM) or BAY11-7082 (6 μM) and then incubated for 30 min. The absorbance at 517 nm was determined using a spectrophotometer. (B) Mice were administered an intraperitoneal bolus of the solvent control (0.1% DMSO), RUT (4 mg/kg) or BAY11-7082 (4 mg/kg), or combination of RUT (2 mg/kg) with BAY11-7082 (2 mg/kg), the ADP (700 mg/kg) was then injected into mouse tail vein. The mortality rate of all groups was observed within 10 min after injection. (C) The bleeding time was measured through mouse tail transection after 30 min of intraperitoneal administration of the solvent control (0.1% DMSO), RUT (4 mg/kg) or BAY11-7082 (4 mg/kg), or combination of RUT (2 mg/kg) with BAY11-7082 (2 mg/kg). Data are presented as the mean ± standard error of the mean ((A), n = 4; (B,C), n = 12).