Table 2.
Antiviral activity of the NBCoV small molecules in a single-cycle assay in different cell lines against pseudovirus NL4-3ΔEnv-NanoLuc/SARS-CoV (IC50) and selectivity index (SI).
| Compound | 293T/ACE2 Cells | HT1080/ACE2 Cells | A549/ACE2 Cells | |||
|---|---|---|---|---|---|---|
| IC50 (nM) a | SI | IC50 (nM) a | SI | IC50 (nM) a | SI | |
| NBCoV1 | 17 ± 2.6 | 2265 | 39 ± 8.2 | 2282 | 98 ± 4 | 878 |
| NBCoV2 | 13.8 ± 0.2 | 2717 | 19.3 ± 1.1 | >5181 | 111 ± 9 | >901 |
| NBCoV3 | 17.8 ± 4 | 2528 | 25.7 ± 0.6 | >3891 | 157 ± 12.5 | >637 |
| NBCoV4 | 80 ± 2 | 509 | 54 ± 3.5 | >1852 | 100 ± 13 | >1000 |
| NBCoV5 | 1853 ± 179 | 19 | 1760 ± 330 | >57 | >2000 | N/A b |
| NBCoV6 | 175 ± 7 | 229 | 133 ± 9.5 | >752 | 867 ± 62 | >115 |
| NBCoV7 | 111 ± 1.7 | 405 | 52 ± 8.9 | >1923 | 271 ± 76 | >369 |
| NBCoV8 | 128 ± 6.7 | 302 | 225 ± 26 | >444 | 700 ± 70 | >143 |
| NBCoV9 | 217 ± 22 | 155 | 246 ± 10 | >407 | 350 ± 15 | >286 |
a The reported IC50 values represent the means ± standard deviations (n = 3). b Not Available.