Table 5.

Microalgae cultivation conditions.

Parameters	Conditions
Temperature	20 ± 2 °C
pH	8.0 ± 0.2
Salinity	33 ± 1‰
Light intensity	300 μmol∙m-2∙s-1, cool white fluorescent
Light cycle	12:12 h light:dark
Cultivation chamber	250 mL Erlenmeyer flask
Age of test organisms	14–20 d, exponential growth phase
Initial bioassay cell density	1.2–1.4 × 104 cells mL−1
Control/diluent water	f/2 medium/0.22 µm filtered seawater