Table 6.

Bioassay endpoints and registration parameters.

Endpoint	Fluorescent Dye or Registered Parameter	Dye Concentration/Duration of Staining *	Instrument	Excitation Source (nm)	Emission Channel/ Band Width (nm)
Growth inhibition	PI	15 µM/10 min	CytoFLEX	488	610/20
Size	Forward scatter intensity (size calibration kit F13838 by Molecular Probes, USA)	–	CytoFLEX	488	FSC
Esterase activity	FDA	50 µM/30 min	GloMax	490	510–570
Membrane potential	DiOC6	7.5 µM/10 min	GloMax	490	510–570
ROS generation	H2DCFDA	250 µM/40 min	GloMax	490	510–570

ROS = reactive oxygen species; PI = propidium iodide; FDA = fluorescein diacetate; DiOC6, 3,3′ = dihexyloxacarbocyanine iodide; H₂DCFDA, 2′,7′ = dichlorodihydrofluorescein diacetate. * For PI, concentration and duration of staining optimization were described in our previous work [46]. The staining parameters for FDA, DiOC₆, and H₂DCFDA were optimized for the GloMax microplate reader by a series of measurements, which included a broad range of concentrations and registration after every 2 min.