FIGURE 1.

YTHDF2 is essential for male mouse fertility. (A) Immunofluorescent staining of YTHDF2 in adult testis. Scale bar, 50 μm. I‐XII indicates the cycle of the seminiferous epithelium. SG, spermatogonia; Pre‐L, pre‐leptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; D, diplotene spermatocyte; RS, round spermatid; ES, elongated spermatid; st, stage. (B) Schematic diagram for the Ythdf2 targeting strategy. (C) Western blot analysis of YTHDF2 in control and Ythdf2‐vKO testes at different ages. P, postnatal day. (D) Immunofluorescent staining of YTHDF2 in control and Ythdf2‐vKO testes. Scale bar, 50 μm. (E) Litter size per plug from Ythdf2‐vKO and control mice. Data are presented as means ± SD (n ≥ 9 for each group). Significance was calculated with unpaired two‐tailed Student's t‐test (** p < 0.01)