FIGURE 6.

YTHDF1 driving the phase separation of YTHDF1/AGO2 complex. (A) AGO2^EGFP (10–40 μM) slightly increased the LLPS in the presence of YTHDF1^mCherry (10 μM) in 150 mM NaCl. (B) YTHDF1^mCherry (10–40 μM) increased the LLPS significantly in the presence of AGO2^EGFP (10 μM) in 150 mM NaCl. (C) RNA^m6a (1–4 μM) did not significantly increase the LLPS of AGO2^EGFP (20 μM) in 150 mM NaCl. (D) RNA^m6A (1–4 μM) increased the LLPS of YTHDF1^mCherry (20 μM) significantly in 150 mM NaCl. (E) RNA^m6A (1–4 μM) increased the LLPS of solutions containing AGO2^EGFP (20 µM) and YTHDF1^mCherry (20 µM) in 150 mM NaCl