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. 2021 Dec 28;14(1):74. doi: 10.3390/pharmaceutics14010074

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SLN.05 or SLN.06 cargo release inside the cells. Fluorescence leakage assay using ANTS/DPX was used to determine cargo delivery and release by the SLN.05 or SLN.06 inside the cells. (A) Schematic summary of fluorescence leakage assay using ANTS/DPX. (B) Schematic of experimental design for fluorescence leakage assay. (C) Micrographs of ARPE-19 cells exposed to ANTS/DPX-loaded SLN.05 and SLN.06. Released ANTS (green) was detectable only in cells exposed to ANTS/DPX loaded into SLNs, and not in cells exposed to free ANTS and/or DPX. Nuclei of cells were stained with DAPI in blue. Scale bar: 10 µm.