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. 2022 Jan 16;23(2):958. doi: 10.3390/ijms23020958

Figure 1.

Figure 1

Lipocalin 2 and pro-inflammatory cytokines expression in MDX mice. (A) ELISA assay showing Lipocalin 2 (Lcn2) serum levels in WT and MDX mice at the ages indicated in the abscissa. (B) Transcriptional expression of Lcn2 in the diaphragm (Diaph), quadriceps (Quad), soleus (Sol), and Extensor Digitorum Longus (EDL) muscles from 1-month-old WT and MDX mice. (C) Immunohistochemical analysis of Lcn2 in diaphragm and quadriceps muscle sections from 1-month-old WT and MDX mice (Black arrowhead: mononuclear cells positive for Lcn2; white arrowhead: positivity for Lcn2 in muscle fibres; bar = 25 μm; inset: magnification of Lcn2-positive mononuclear cells). (D) ELISA assay to evaluate the serum levels of Interleukin (IL) 6 and (E) IL1β at the ages indicated in the abscissa. (F) Diaphragm, quadriceps, soleus (Sol), and Extensor Digitorum Longus (EDL) transcriptional expression of Il6, (G) Il1b, and (H) Tnfa in 1-month-old WT and MDX mice. In (C), pictures are representative of three mice/group. In (A,B,D–H) results are the mean ± SD of at least three mice/group; N.D. = non-detectable; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. WT; Student’s t-test.