Figure 3.
AntiGan regulates COX-2 and IL-17 expression in cancer cells in vitro. HepG2 (A) and HCT116 (B) cells were treated with AntiGan (10 and 50 µg/mL) for 48 h. The cells were immunolabeled with antibodies against COX-2 (red) and IL-17 (green). A, A’, D, D’, Control cells; B, B’, E, E’, ANTIGAN 10 ug/mL; C, C’, F, F’, ANTIGAN 50 ug/ml. Cells were counterstained with DAPI (blue) to label nuclei. Apostrophe indicates DAPI staining COX-2, cyclooxygenase-2; IL-17, interleukin 17. Scale bar, 100 µm for all images.