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. 2022 Jan 20;15:11. doi: 10.1186/s13048-022-00946-w

Table 1.

Primer sequences used for real time PCR analysis

Gene (Mus musculus) Forward primer(5′-3′) Reverse primer(5′-3′) Annealing temperature(°C) Product size (bp)
Gdf9 AAAGAAGACTGGCACGAGGA CAAAGCAGCAAACCCCCAA 59 92
Bmp15 TGGGTTCCTAAATGCCGGAC TGCCTTTTTGCTGCTGACAC 60 181
Gpx1 TGCAATCAGTTCGGACACCA TCTCACCATTCACTTCGCACT 59 130
Sod1 AGCATTCCATCATTGGCCGT TCCCAGCATTTCCAGTCTTTGT 60 99
Bcl2 AATTGTAATTCATCTGCCGCCG GTGGAGGAAAAATCAGGAGGGT 60 150
Bax ACATGGCAGACAGTGACCAT AAGACACAGTCCAAGGCAGT 59 103
β-actin AAGTACTCTGTGTGGATCGGTG GGTGTAAAACGCAGCTCAGTAA 59 153