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. 2021 Dec 27;10(1):32. doi: 10.3390/vaccines10010032

Figure 1.

Figure 1

Gene cloning and vector construction. (A) RT-PCR amplification of ntmEmROM5. M, DNA molecular weight standard of DL5000. Lane 1, amplification products of ntmEmROM5 (963 bp). (B) RT-PCR amplification of EmROM5. M, DNA molecular weight standard of DL2000. Lane 2, amplification products of EmROM5 (1461 bp). (C) Enzyme digestion identification of pET-32a–ntmEmROM5. M, DNA molecular weight standard of DL2000. Lane 3, enzyme digestion identification of pET-32a–ntmEmROM5 (963 bp). (D) Enzyme digestion identification of pVAX–EmROM5. M, DNA molecular weight standard of DL5000. Lane 4, enzyme digestion identification of pVAX-EmROM5 (1461 bp).