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. 2022 Jan 21;22:88. doi: 10.1186/s12885-022-09194-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Polarization of macrophages can be facilitated by EVs from cervical cancer patients before and after radiotherapy. Peripheral blood mononuclear cells were obtained by Ficoll-Plaque density gradient centrifugation from healthy donors. Monocytes were isolated and cultured in RPMI 1640 medium. M2-polarized macrophages were obtained by IL-4+IL-13 stimulation for 48 hours. A, flow cytometry analysis the expression levels of cell surface and intracellular markers in macrophages before and after M2 polarization. M2 polarized macrophages were treated with EVs (the particle number per milliliter was 5 times higher than that in plasma) from cervical cancer patients before and after radiotherapy. B, flow cytometry analysis the expression levels of cell surface and intracellular markers. The dose effects of EVs on the expression levels of CD163 (C) and CCR7 (D) in M2 polarized macrophages were shown (n = 4). Bars presented as mean values of indicated markers. *, p < 0.05; **, p < 0.01. P value was calculated by two-tailed Mann Whitney U test