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. 2022 Jan 21;22:88. doi: 10.1186/s12885-022-09194-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — EVs from cervical cancer patients after radiotherapy contribute to increased macrophage phagocytosis. Peripheral blood mononuclear cells from healthy donors were obtained and cultured in RPMI 1640 medium. M2-polarized macrophages were obtained by IL-4+IL-13 stimulation and treated with EVs from cervical cancer patients before and after radiotherapy. A, representative scatter diagrams of flow cytometry analysis the expression of PD-1 and CCR7 in macrophages (CD45⁺CD14⁺CD11b⁺) were shown. Histograms of PD-1⁺ cells in total macrophages (B) and in CCR7⁺ macrophages (C) were shown. Macrophage phagocytosis was assessed using pHrodo Red E. coli bioparticles or CFSE labeled EVs. Representative diagram (D) and histogram (E) about mean fluorescent intensity (MFI) of pHrodo in macrophages were shown. Representative scatter diagram (F) and histogram (G) of flow cytometry analysis the correlation between PD-1 expression and MFI of pHrodo in macrophages were shown. Representative diagram (H) and histogram (I) of CFSE MFI in macrophages co-cultured with EVs labeled with or without (Control) CFSE, after treated with EVs from cervical cancer patients before and after radiotherapy for 24 hours, were shown. Data were presented as mean±SD. P value was calculated by two-tailed Mann Whitney U test