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General scheme of bioconjugation reactions for the obtention of a drug-loaded nanobiohybrid carrier. Step A: First, a chemical group R1 is incorporated in the surface of bacteria. Step B: Nanoparticles are functionalized with the chemical partner of R1 (R2). Step C: Then, NPs are loaded with the desired therapeutic agent. Step D: Bioconjugation reaction between the chemical partners R1 and R2 leads to the bioconjugate bacteria-nanomaterial.
