Fig. 6.

Effect of DBPR114 on gene expression profiles in Huh7 xenograft tumors. A Heatmap displaying the differentially expressed genes in DBPR114-treated tumors. Huh7 tumors from the control and treated groups (Fig. 4) were harvested at the end of study. Tumor tissue RNA was isolated and subjected to RNA sequencing analysis using human-specific genome read data. The significantly upregulated and downregulated genes between the treatment and vehicle control groups were selected if they had an FDR < 0.05 and absolute fold change ≥ 2. n = 4 per group for vehicle control, and n = 3 per group for DBPR114. Red: increased; blue: decreased; white: unchanged. B GSEA (FDR < 0.05) revealed enrichment of the gene sets of the G2/M checkpoint, mitotic spindle assembly, E2F targets, and mTORC complex 1 signaling following DBPR114 treatment