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. 2022 Jan 20;22:17. doi: 10.1186/s12906-021-03477-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A&P inhibits inflammaion in BMDM cells. a-b, MTT viability assay. c-f, Effect of A&P on the mRNA expression of Mincle, TNF-α, IL-1β and IL-6 in HG-induced BMDM cells according to Real-time PCR analysis. g-i, the content of TNF-α, IL-1β and IL-6 in supernatant in HG-induced BMDM cells detected by ELISA assay. j, The relative expression of p-NFκB detected by western boltting analysis normalized to NFκB expression, and the relative expression of Mincle, Card9, TNF-α, IL-1β and IL-6 detected by western boltting analysis normalized to β-actin expression. (The data are expressed as the mean ± SD. Compared with Control group, *P < 0.05. Compared with HG group, ^#P < 0.05, ^##P < 0.01)