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. 2022 Jan 20;22:17. doi: 10.1186/s12906-021-03477-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — HG-induced BMDM aggravated the inflammatory response of MES cells. a, The schematic diagram of the co-culture system. b-d, Effect of A&P on the mRNA expression of TNF-α, IL-1β and IL-6 in HG-induced MES cells and co-culture cells detected by Real-time PCR analysis. e-i, Effect of A&P on the protein level of p-NFκB, TNF-α, IL-1β and IL-6 in HG-induced MES cells and co-culture cells detected by western blotting analysis. (The data are expressed as the mean ± SD. Compared with Control group of single culture, *P < 0.01. Compared with HG group of single culture, ^#P < 0.01. Compared with HG group of single culture, **P < 0.01. Compared with HG group of co-culture, ^#P < 0.01)