Figure 3.
Naringenin induces reactive oxygen species (ROS) generation in human osteosarcoma cells. (A,B) Cells were treated with naringenin (100, 250, and 500 μM) for 1 h and stained with H2DCFDA. The percentage of ROS production was determined by flow cytometry. (C) Cells were treated as described in A-B. ROS inhibitors inhibited ROS production. (D) After cells were pretreated with a ROS inhibitor for 1 h, followed by stimulation with naringenin for 24 h, cell viability was examined by using the CCK-8 assay. Results are expressed as the mean ± SD of three independent experiments. * p < 0.05 compared with the control group; # p < 0.05 compared with the naringenin-treated group.