Table 1. Anti-Leishmania activity, cytotoxicity, and selectivity index of 8HQN and AmB.

Growth of parasites and viability of THP-1 cells were determined using alamarBlue assay. The results were expressed as mean ± SD. The selectivity index (SI) was calculated by the ratio between the CC₅₀ and IC₅₀ values, respectively.

Biological activity
Compound (µg/mL)	Promastigotes	Amastigotes	THP-1 cells
	IC50a/ SId	IC50b/SId	CC50c
8HQN	1.61 ± 0.28/79.84	1.56 ± 0.02/82.40	128.55 ± 0.92
AmB	0.04 ± 0.02/23.75	0.04 ± 0.01/23.75	0.95 ± 0.32

Notes:

^aInhibitory concentration for 50% of promastigotes.

^bInhibitory concentration for 50% of amastigotes.

^cCytotoxic concentration for 50% of THP-1 cells.

^dSelectivity index.