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. 2022 Jan 21;13:22. doi: 10.1186/s13287-022-02702-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CB1 promoted the osteogenic differentiation of hBMSCs under TNF-α and INF-γ stimulation. A–D 10 ng/ml TNF-α was used to treat BMSCs. A Real-time RT-PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 10 ng/ml TNF-α treatment in BMSCs. B ALP activity assay. C Alizarin red staining. D Calcium quantitative analysis. E–H 100 ng/ml INF-γ was used to treat BMSCs. E Real-time RT-PCR results showed the expression of CB1 at 1, 2, 4 and 8 h after 100 ng/ml INF-γ treatment in BMSCs. F ALP activity assay. G Alizarin red staining. H Calcium quantitative analysis. GAPDH was used as an internal control. One-way ANOVA was performed to determine statistical significance. Error bars represent the SD (n = 3). *P ≤ 0.05; **P ≤ 0.01