Figure 3.

Relative contributions of synonymous, non-synonymous and nonsense tandem dinucleotide substitutions for VDJ, VJ-kappa and VJ-lambda. (A) Results are split per position in the codon. Position 1 represents 5’ and middle base on the coding strand, position 2 represents middle and 3’ base and position 3 represents the 3’ base of the codon and the 5’ base of the downstream codon. Tandem dinucleotide substitutions causing two separate synonymous mutations in position 3 are displayed as a separate category. (B) Analysis of AID motifs present in tandem substitution for IGHV sequences. The 3 motifs associated with AID activity (WRCY, WA, and RCG) were identified in position 5’ or 3’ of the tandem. The motifs were considered in forward (coding) and reverse (template) direction.