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. 2022 Jan 5;14(1):124. doi: 10.3390/pharmaceutics14010124

Figure 5.

Figure 5

Anti-tumor effect on GL261 tumor-bearing mice model treated with DOX and HA-DOX. (A) C57BL/6J mice were treated with PBS, DOX, HA-NH-DOX, HA-Hz-DOX, and HA-AdpHz-DOX conjugates (3 mg/kg in DOX equivalent) after 10 days following tumor transplantation. Tumor growth was delayed in the HA-Hz-DOX group (green); in the PBS (grey) and the DOX (red) groups, the tumor growth was higher in comparison to the groups treated with the HA-DOX conjugates; in the HA-NH-DOX (blue) and the HA-AdpHz-DOX (violet) groups, the tumor growth rate was overlapped. Each point represents the mean of tumor size. (B) Comparison of tumor distribution at day 25, indicating that HA-Hz-DOX was statistically significant compared to untreated and DOX. (C) Kaplan–Meier curves: HA-Hz-DOX and HA-AdpHz-DOX groups presented the highest survival rate. (Mean, error bar = SEM, N = 5, statistical analysis obtained by one-way ANOVA multiple comparison: ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001).