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. 2022 Jan 13;9(1):27. doi: 10.3390/jcdd9010027

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Effects of VitD on IL-6-induced ACE2r transcription in human endothelial cells assessed by Real Time quantitative PCR. ACE2r mRNA was undetectable at baseline. Incubation with IL-6 caused significant increase in ACE2r mRNA levels, as compared to unstimulated cells. Preincubation with VitD inhibited the IL-6 effect on ACE2r-mRNA. LPS served as positive control. Data are expressed as fold induction versus control gene represented by GAPDH. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. oxLDL; one-way ANOVA with Tukey’s post hoc test). (B) Effects of VitD on IL-6-induced ACE2r protein evaluated by Western blot analysis of cell lysates. IL-6 caused a significant increase of ACE2r protein levels. These effects were prevented by treatment with VitD. Tubulin served as loading control. LPS served as positive control. Each bar represents the mean ± SD of 6 different experiments (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6; one-way ANOVA with Tukey’s post hoc test). The insert shows results of a representative experiment.