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. 2022 Jan 13;9(1):27. doi: 10.3390/jcdd9010027

Figure 5.

Figure 5

(A) In HUVEC incubated with IL-6, a significant increase of NF-kB levels was observed. Pretreatment of endothelial cells with VitD prevented the IL-6 effects on NF-kB. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test). (B) Western blot analyses. IL-6 stimulation results in significant reduced levels of IκB. Conversely, preincubation with VitD restores IκB levels. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test). The insert shows results of a representative experiment. (C) In HUVEC incubated with IL-6, a significant increase of STAT3 levels was observed. Pretreatment of endothelial cells with VitD prevented the IL-6 effects on STAT3. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test).