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. 2022 Jan 6;11(2):148. doi: 10.3390/plants11020148

Table 1.

Induction of hairy roots (HRs) from petiole and leaf explants of Centella asiatica using a 3-week-old culture of Agrobacterium rhizogenes strain KCCM11879.

Explant Treatment No. of Explants Survival Rate (%) Frequency of Root Formation (%) No. of Confirmed HR z Lines
Leaf Control 50 100 ± 0.0 a y 4.0 ± 0.1 b 0.0 ± 0.0 b
KCCM 11879 250 100 ± 0.0 a 26.8 ± 2.7 a 19.1 ± 0.7 a
Petiole Control 90 100 ± 0.0 a 0.0 ± 0.1 b 0.0 ± 0.0 b
KCCM 11879 450 100 ± 0.0 a 12.0 ± 1.6 a 17.0 ± 1.9 a

z Confirmed hairy root by rol gene detection using PCR; y Different letters indicate significant differences at p < 0.05 according to Duncan’s multiple range test.