Table 3.
Effects of hemodialysis on total plasma oxylipins and their ratios in the CKD patients before (pre-HD) and at cessation (post-HD) of hemodialysis (n = 12 each).
| Amount ng/ml | Pre-HD A (Mean ± SD) |
Pre-HD V (Mean ± SD) |
p-Value, t-Test (# Paired Wilcoxon test) |
Pre-HD A-V Difference (Mean ± SD) |
Post-HD A (Mean ± SD) |
Post-HD V (Mean ± SD) |
p-Value, t-test (# Paired Wilcoxon Test) |
Pre-HD A-V Difference (Mean ± SD) |
|---|---|---|---|---|---|---|---|---|
| 5,6-EET + 5,6-DHET | 26.3853 ± 22.7488 | 17.8740 ± 15.3357 | 0.325 # | 8.2771 ± 22.0947 | 38.4409 ± 16.7998 | 63.1169 ± 21.0481 | 0.003 # | −24.6760 ± 17.5393 |
| 8,9-EET + 8,9-DHET | 12.2661 ± 10.8593 | 8.8769 ± 6.7288 | 0.758 # | 3.2897 ± 11.3985 | 14.9171 ± 6.2221 | 23.6504 ± 7.8856 | 0.003 # | −8.7333 ± 7.0409 |
| 11,12-EET + 11,12-DHET | 10.3905 ± 10.0509 | 7.4546 ± 6.8226 | 0.902 # | 2.7353 ± 10.2061 | 14.7525 ± 6.7118 | 26.1620 ± 9.1754 | 0.002 # | −11.4095 ± 7.5222 |
| 14,15-EET + 14,15-DHET | 12.9774 ± 12.5015 | 10.0376 ± 9.5061 | 0.667 # | 2.6766 ± 12.4408 | 19.5791 ± 9.2852 | 36.1083 ± 13.6146 | 0.002 # | −16.5292 ± 10.9098 |
| 7,8-EDP + 7,8-DiHDPA | 4.9725 ± 4.3744 | 3.7040 ± 3.1733 | 0.854 # | 1.2292 ± 4.8036 | 6.4969 ± 2.8052 | 10.8874 ± 3.5963 | 0.002 # | −4.3904 ± 2.2808 |
| 10,11-EDP + 10,11-DiHDPA | 4.6062 ± 4.3839 | 3.4580 ± 3.0889 | 0.806 # | 1.0598 ± 4.5591 | 6.7795 ± 3.0931 | 12.0623 ± 3.9527 | <0.001 | −5.2828 ± 2.5347 |
| 13,14-EDP + 13,14-DiHDPA | 3.4752 ± 3.4913 | 2.7467 ± 2.8205 | 0.854 # | 0.6542 ± 3.6762 | 4.9215 ± 2.2192 | 7.9218 ± 2.6423 | <0.001 | −3.0002 ± 1.9422 |
| 16,17-EDP + 16,17-DiHDPA | 3.0618 ± 2.8699 | 2.2873 ± 2.0344 | 0.712 # | 0.7529 ± 2.8441 | 3.9502 ± 1.7125 | 5.6394 ± 2.0354 | 0.009 | −1.6892 ± 1.8365 |
| 19,20-EDP + 19,20-DiHDPA | 6.3937 ± 5.2944 | 4.7947 ± 3.1021 | 1 # | 1.5121 ± 5.6027 | 9.6295 ± 4.0652 | 17.2258 ± 6.4814 | <0.001 | −7.5963 ± 3.8630 |
| 5,6-EEQ + 5,6-DiHETE | 1.5350 ± 0.9296 | 1.3729 ± 0.7133 | 0.622 # | 0.3217 ± 0.8716 | 2.0440 ± 1.5009 | 1.7043 ± 0.8338 | 0.388 # | 0.3397 ± 1.2857 |
| 8,9-EEQ + 8,9-DiHETE | 1.5386 ± 1.2263 | 1.0880 ± 0.6966 | 0.285 | 0.4408 ± 1.3934 | 2.7148 ± 1.8146 | 4.4861 ± 1.6000 | 0.008 # | −1.7714 ± 1.6800 |
| 11,12-EEQ + 11,12-DiHETE | 1.0017 ± 0.8179 | 0.7552 ± 0.4846 | 0.758 # | 0.2422 ± 0.9263 | 1.8673 ± 1.2279 | 3.5323 ± 1.3814 | 0.003 # | −1.6651 ± 1.1940 |
| 14,15-EEQ + 14,15-DiHETE | 0.9963 ± 0.7071 | 0.7508 ± 0.4514 | 0.328 | 0.2305 ± 0.8200 | 1.8082 ± 1.2226 | 3.3214 ± 1.3339 | 0.004 # | −1.5133 ± 1.3083 |
| 17,18-EEQ + 17,18-DiHETE | 1.8998 ± 1.3591 | 1.6056 ± 0.8496 | 0.536 | 0.2613 ± 1.5047 | 3.2405 ± 2.1560 | 6.5059 ± 2.3420 | 0.003 # | −3.2654 ± 2.0580 |
| 9,10-EpOME + 9,10-DiHOME | 37.1683 ± 31.3783 | 25.8981 ± 13.6496 | 0.712 # | 10.7144 ± 32.1530 | 57.3217 ± 18.9513 | 99.2342 ± 38.1881 | 0.002 # | −41.9125 ± 28.5066 |
| 12,13-EpOME + 12,13-DiHOME | 35.9634 ± 27.1475 | 24.5992 ± 12.1650 | 0.424 # | 10.7813 ± 27.9218 | 53.8703 ± 15.3169 | 86.6564 ± 25.6785 | <0.001 | −32.7861 ± 16.4280 |
| 5,6-DHET/5,6-EET | 0.0749 ± 0.0518 | 0.0864 ± 0.0565 | 0.617 | 0.0431 ± 0.0261 | 0.0222 ± 0.0120 | 0.002 # | ||
| 8,9-DHET/8,9-EET | 0.2737 ± 0.2578 | 0.2858 ± 0.2519 | 0.854 # | 0.1253 ± 0.0630 | 0.0599 ± 0.0480 | 0.002 # | ||
| 11,12-DHET/11,12-EET | 0.0827 ± 0.0649 | 0.0874 ± 0.0650 | 0.926 # | 0.0426 ± 0.0205 | 0.0198 ± 0.0099 | 0.002 # | ||
| 14,15-DHET/14,15-EET | 0.0654 ± 0.0409 | 0.0550 ± 0.0284 | 0.485 | 0.0326 ± 0.0210 | 0.0127 ± 0.0047 | 0.002 # | ||
| 7,8-DiHDPA/7,8-EDP | 0.1650 ± 0.1420 | 0.5745 ± 1.4719 | 0.622 # | 0.0892 ± 0.0353 | 0.0458 ± 0.0285 | 0.002 # | ||
| 10,11-DiHDPA/10,11-EDP | 0.0538 ± 0.0350 | 0.0514 ± 0.0285 | 0.859 | 0.0256 ± 0.0118 | 0.0110 ± 0.0041 | 0.002 # | ||
| 13,14-DiHDPA/13,14-EDP | 0.0506 ± 0.0302 | 0.0484 ± 0.0269 | 0.855 | 0.0237 ± 0.0101 | 0.0134 ± 0.0039 | 0.01 # | ||
| 16,17-DiHDPA/16,17-EDP | 0.0552 ± 0.0392 | 0.0601 ± 0.0343 | 0.755 | 0.0335 ± 0.0139 | 0.0194 ± 0.0071 | 0.008 | ||
| 19,20-DiHDPA/19,20-EDP | 0.2730 ± 0.2232 | 0.2905 ± 0.1983 | 0.806 # | 0.1212 ± 0.0480 | 0.0580 ± 0.0264 | 0.002 # | ||
| 5,6-DiHETE/5,6-EEQ | 483.3002 ± 348.2197 | 599.0058 ± 431.5977 | 0.49 | 274.7175 ± 163.7055 | 121.8920 ± 63.0904 | 0.001 | ||
| 8,9-DiHETE/8,9-EEQ | 0.1037 ± 0.0654 | 0.1332 ± 0.0872 | 0.372 | 0.0502 ± 0.0261 | 0.0229 ± 0.0107 | 0.002 # | ||
| 11,12-DiHETE/11,12-EEQ | 0.1107 ± 0.1548 | 0.0634 ± 0.0260 | 0.806 # | 0.0596 ± 0.1111 | 0.0112 ± 0.0032 | 0.002 # | ||
| 14,15-DiHETE/14,15-EEQ | 0.1012 ± 0.0933 | 0.0817 ± 0.0318 | 0.854 # | 0.0436 ± 0.0466 | 0.0153 ± 0.0044 | 0.003 # | ||
| 17,18-DiHETE/17,18-EEQ | 0.2320 ± 0.1551 | 0.2159 ± 0.1591 | 0.667 # | 0.1399 ± 0.1461 | 0.0397 ± 0.0133 | 0.002 # | ||
| 9,10-DiHOME/9,10-EpOME | 0.1799 ± 0.0860 | 0.1731 ± 0.1090 | 0.58 # | 0.1260 ± 0.0918 | 0.0617 ± 0.0222 | 0.01 # | ||
| 12,13-DiHOME/12,13-EpOME | 0.2255 ± 0.0981 | 0.2087 ± 0.0951 | 0.356 # | 0.1627 ± 0.1101 | 0.0877 ± 0.0327 | 0.008 # | ||
| Ratio(5,6-DHET+8,9-DHET+11,12-DHET+14,15-DHET) / (5,6-EET+8,9-EET +11,12 EET +14,15-EET) |
0.1104 ± 0.0878 | 0.1188 ± 0.0946 | 1 # | 0.0541 ± 0.0262 | 0.0253 ± 0.0143 | 0.002 # | ||
| Ratio(7,8-DiHDPA+10,11-DiHDPA +13,14-DiHDPA+16,17-DiHDPA+19,20-DiHDPA) / (7,8-EDP+10,11-EDP+13,14-EDP+16,17-EDP+19,20-EDP) |
0.1369 ± 0.1095 | 0.1478 ± 0.1106 | 0.902 # | 0.0666 ± 0.0240 | 0.0336 ± 0.0143 | 0.002 # | ||
| Ratio(5,6-DiHETE+8,9-DiHETE+11,12-DiHETE+14,15-DiHETE+17,18-DiHETE) / (5,6-EEQ+ 8,9-EEQ+11,12-EEQ+14,15-EEQ+17,18-EEQ) |
0.6541 ± 0.4424 | 0.6335 ± 0.4128 | 0.909 | 0.3403 ± 0.2022 | 0.1315 ± 0.0660 | 0.002 # | ||
| Ratio (9,10-DiHOME+12,13-DiHOME) / (9,10-EpOME+12,13-EpOME) |
0.2017 ± 0.0845 | 0.1904 ± 0.1007 | 0.58 # | 0.1445 ± 0.1017 | 0.0741 ± 0.0258 | 0.008 # |
Notes: A, arterial blood; V, venous blood; A-V difference, arteriovenous difference. Bold indicates significant difference.