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Summary of study methods. Stool and clinical evaluation were collected from 129 adults (94 PLWH and 35 HIV−). A subset of participants (32 PLWH vs. 13 HIV−) underwent emotional assessment using the NIH toolbox emotion battery. The hypervariable region V3-V4 was amplified from stool DNA and sequenced using the Illumina MiSeq. Bioinformatics and statistical analyses were performed using the CLC Microbial Genomics Module and R statistical Software, respectively
