. 2022 Jan 1;14(1):24. doi: 10.3390/toxins14010024

Table 2.

Effect of pH on biodegradation of AFB1 by leaf and shoot extracts of Mentha arvensis.

Treatments	pH	3 h		6 h		12 h		24 h		48 h		72 h
Treatments	pH	Toxin Recovery	D%	Toxin Recovery	D%	Toxin Recovery	D%	Toxin Recovery	D%	Toxin Recovery	D%	Toxin Recovery	D%
Toxin AFB1		99 ± 7.8 ^r	0.81	99 ± 6.7 ^r	0.88	98 ± 7.7 ^r	1.80	97 ± 5.4 ^r	2.3	96 ± 6.4 ^q,r	3.07	96 ± 7.5 ^o–r	3.81
Toxin + H₂O	pH2	96 ± 9.5 ^p,q,r	3.2	95 ± 7.3^{n –r}	4.6	92 ± 6.9 ^k–p	7.8	90 ± 6.9 ^g–l	9.6	89 ± 6.5 ^e–l	10.2	87 ± 5.9 ^d–j	12.7
Toxin + H₂O	pH4	96 ± 7.6 ^o–r	3.8	95 ± 7.4 ^n–r	4.7	90 ± 8.8 ^h–m	9.2	88 ± 5.9 ^d–l	11.6	87 ± 8.9 ^d–i	13.0	86 ± 7.9 ^c–h	13.9
Toxin + H₂O	pH6	94 ± 5.2 ^m–r	5.1	92 ± 6.8 ^l–q	7.5	90 ± 6.8 ^f–l	9.8	88 ± 7.4 ^c–l	12.0	86 ± 6.1 ^c–g	14.0	85 ± 6.0 ^b–f	14.9
Toxin + H₂O	pH8	91 ± 6.9 ^j–o	8.1	89 ± 6.3 ^e–l	10.1	87 ± 6.1 ^d–k	12.3	85 ± 5.9 ^b–c	14.7	84 ± 5.9 ^a–d	15.9	83 ± 5.7 ^a–d	16.5
Toxin + H₂O	pH10	90 ± 6.8 ^i–n	8.6	87 ± 6.1 ^d–k	12.2	85 ± 5.0 ^b–c	14.7	82 ± 5.0 ^a,b,c	17.7	81 ± 4.5 ^a,b	18.8	80 ± 5.4 ^a	19.9
Toxin + H₂O	WpH	98 ± 5.7 ^r	1.09	97 ± 5.6 ^r	2.6	96 ± 5.9 ^p,q,r	3.23	96 ± 5.8 ^o–r	3.56	96 ± 5.9 ^o–r	3.97	95 ± 7.4 ^n–r	4.19
Leaf extract + AFB1	pH2	56 ± 3.3 ^h–u	43.8	55 ± 4.4 ^c–u	44.4	52 ± 2.2 ^a–t	47.7	48 ± 3.0^a–o	51.7	42 ± 1.8 ^a–l	57.5	37 ± 1.6 ^a–j	62.2
	pH4	54 ± 2.5 ^b–u	45.7	55 ± 3.5 ^a–u	44.4	52 ± 3.2 ^a–t	47.7	48 ± 1.9 ^a–o	51.7	42 ± 2.9 ^a–j	57.5	37 ± 2.5 ^a–j	62.2
	pH6	36 ± 1.6 ^a–q	64.0	34 ± 2.4 ^a–n	65.2	30 ± 1.6 ^a–l	70.0	27 ± 1.9 ^a–l	72.6	24 ± 0.9 ^a–i	75.9	20 ± 0.6 ^a–e	79.6
	pH8	38 ± 3.7 ^a–n	61.6	34 ± 1.3 ^a–l	65.7	30 ± 1.5 ^a–k	69.4	28 ± 1.1 ^a–j	71.2	23 ± 0.7 ^a–h	76.8	18 ± 0.4 ^a,b,c	81.8
	pH10	30 ± 1.5 ^a–j	69.1	28 ± 1.2 ^a–j	72.0	26 ± 1.9 ^a–h	73.9	23 ± 0.8 ^a–g	76.4	17 ± 1.5 ^a,b	82.4	12 ± 0.3 ^a	87.6
	WpH	57 ± 3.4 ^i–u	42.5	56 ± 3.3^d-u	43.6	53 ± 3.4 ^b–t	46.3	50 ± 4.2 ^a–q	49.8	45 ± 3.1 ^a–m	54.4	40 ± 1.9 ^a–l	59.3
Shoot extract + AFB1	pH2	75 ± 4.7 ^q–u	24.7	76 ± 4.6 ^r–u	23.7	75 ± 6.5 ^q–u	24.2	72 ± 5.5 ^k–u	27.2	66 ± 4.3 ^e–u	34.0	61 ± 3.6 ^d–u	38.1
	pH4	77 ± 5.8 ^p–u	22.6	76 ± 4.7 ^p–u	23.4	76 ± 4.7 ^o–u	24.0	69 ± 4.2 ^f–u	30.8	68 ± 3.5 ^e–u	31.2	63 ± 5.5 ^d–u	36.3
	pH6	74 ± 4.3 ^k–u	25.9	73 ± 6.2 ^j–u	26.7	71 ± 4.3 ^g–u	28.5	69 ± 3.2 ^f–u	30.3	67 ± 3.8 ^e–u	32.4	62 ± 2.5 ^d–u	37.1
	pH8	67 ± 3.8 ^e–u	32.1	66 ± 3.9 ^e–u	33.6	65 ± 2.6 ^e–u	34.6	64 ± 4. 1^d–u	35.8	60 ± 3.5 ^b–u	39.3	57 ± 3.2 ^a–u	42.8
	pH10	61 ± 4.7 ^d–u	38.79	60 ± 4.5 ^d–u	39.2	58 ± 3.4 ^d–u	42.3	56 ± 3.2 ^d–u	43.4	50 ± 4.1 ^a–q	49.4	43 ± 2.9 ^a–m	56.9
	WpH	73 ± 4.4 ^j–u	26.2	71 ± 4.5 ^g–u	28.8	68 ± 3.8 ^f–u	31.4	65 ± 5.1 ^e–u	34.6	62 ± 3.7 ^f–u	37.3	59 ± 4.3 ^j–u	40.2

Data were analyzed by analysis of variance (ANOVA). Letters in uppercase indicate significant differences (p < 0.05) as calculated by Tukey’s Multiple Range test. D%: Degradation percentage. WpH: without pH adjustment.