Table 1.
Gene | Primer Orientation | Nucleotide Sequence |
---|---|---|
b-actin | forward | 5′-AGAGCTATGAGCTGCCTGACG-3′ |
reverse | 5′-CCGCAAGATTCCATACCCA-3′ | |
Inflammatory pathway genes |
||
TNF-α | forward | 5′-GCTGGATCTTCAAAGTCGGGTGTA-3′ |
reverse | 5′-TGTGAGTCTCAGCACACTTCCATC- -3′ | |
Il-1β | forward | 5′-TGGACTTCGCAGCACAAAATG-3′ |
reverse | 5′-GTTCACTTCACGCTCTTGGATG-3′ | |
Il-6 | forward | 5′- -AGACCGCTGCCTGTCTAAAA-3′ |
reverse | 5′-TTTGATGTCGTTCACCAGGA-3′ | |
Il-8 | forward | 5′-GTCGCTGCATTGAAACAGAA- -3′ |
reverse | 5′-CTTAACCCATGGAGCAGAGG-3′ | |
NF-κB | forward | 5′-GAGCCCTTTGTGCAAGAGAC-3′ |
reverse | 5′-TGGGATACGTCCTCCTGTTC-3′ | |
IκBα | forward | 5′-TTTCGGAGGAGATGGAGAGA-3′ |
reverse | 5′-CTGTTCAGGTACGGGTCGTT-3′ | |
Apoptosis pathway genes | ||
cas-3 | forward | 5′-CCGCTGCCCATCACTA-3′ |
reverse | 5′-ATCCTTTCACGACCATCT-3′ | |
Bax | forward | 5′-GGCTATTTCAACCAGGGTTCC-3′ |
reverse | 5′-TGCGAATCACCAATGCTGT-3′ | |
bcl-2 | forward | 5′-TCACTCGTTCAGACCCTCAT-3′ |
reverse | 5′-ACGCTTTCCACGCACAT-3′ |