Skip to main content
. 2022 Jan 17;12(1):128. doi: 10.3390/life12010128

Table 1.

Primers for Real-Time PCR.

Gene Primer Orientation Nucleotide Sequence
b-actin forward 5′-AGAGCTATGAGCTGCCTGACG-3′
reverse 5′-CCGCAAGATTCCATACCCA-3′
Inflammatory
pathway genes
TNF-α forward 5′-GCTGGATCTTCAAAGTCGGGTGTA-3′
reverse 5′-TGTGAGTCTCAGCACACTTCCATC- -3′
Il-1β forward 5′-TGGACTTCGCAGCACAAAATG-3′
reverse 5′-GTTCACTTCACGCTCTTGGATG-3′
Il-6 forward 5′- -AGACCGCTGCCTGTCTAAAA-3′
reverse 5′-TTTGATGTCGTTCACCAGGA-3′
Il-8 forward 5′-GTCGCTGCATTGAAACAGAA- -3′
reverse 5′-CTTAACCCATGGAGCAGAGG-3′
NF-κB forward 5′-GAGCCCTTTGTGCAAGAGAC-3′
reverse 5′-TGGGATACGTCCTCCTGTTC-3′
IκBα forward 5′-TTTCGGAGGAGATGGAGAGA-3′
reverse 5′-CTGTTCAGGTACGGGTCGTT-3′
Apoptosis pathway genes
cas-3 forward 5′-CCGCTGCCCATCACTA-3′
reverse 5′-ATCCTTTCACGACCATCT-3′
Bax forward 5′-GGCTATTTCAACCAGGGTTCC-3′
reverse 5′-TGCGAATCACCAATGCTGT-3′
bcl-2 forward 5′-TCACTCGTTCAGACCCTCAT-3′
reverse 5′-ACGCTTTCCACGCACAT-3′