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IF decreases cardiac m6A methylation in HFD-fed mice. (A) The m6A level in heart tissue samples. (B) RT-PCR analysis of the mRNA levels of m6A methylation-related genes in cardiac tissues related to writer (METTL3, METTL14, and WTAP), eraser (FTO and ALKBH5), and reader (YTHDF1, YTHDF2, YTHDF3, YTHDC1, and YTHDC2). (C,D) Western blot images and their densitometric quantitative analysis of METTL3, FTO, and YTHDF1. Data are shown as means ± SEM. * p < 0.05, ** p < 0.01.
