Table 1.
Major identified compounds in F3 and F4 fractions of E. crinita and their tentative identification by UHPLC-ESI(+)-HRMS.
| No. | Compound | Elemental Composition | [M + H]+ | Error ** (ppm) |
A | B | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| RT (min) | Area (Counts) | RT (min) | Area (Counts) | |||||||
| F3 | F4 | F3 | F4 | |||||||
| 1. | Palmitoleamide | C16H31NO | 254.24784 | 0.5 | 13.609 | 1108116 | 320801 | 13.085 | 1309202 | 3128998 |
| 2. | Linoleamide | C18H33NO | 280.26349 | 0.7 | 14.086 | 1390454 | 3166360 | 13.546 | 1574298 | 3629020 |
| 3. | Palmitamide | C16H33NO | 256.26349 | 0.9 | 14.460 | 5498614 | 3834048 | 13.818 | 6396427 | 3991527 |
| 4. | 2,3-Dihydroxypropyl palmitate | C19H38O4 | 331.28429 | 4.0 | 14.817 | 238983 | 16797 | 14.129 | 295785 | 21659 |
| 5. | Oleamide | C18H35NO | 282.27914 | 0.5 | 14.868 | 25284826 | 54228974 | 14.209 | 26096208 | 53855452 |
| 6. | 2,3-Dihydroxypropyl stearate | C21H42O4 | 359.31559 | 4.0 | 15.974 | 330917 | 58977 | 15.162 | 409784 | 70222 |
| 7. | Erucamide | C22H43NO | 338.34174 | 0.1 | 16.981 | 5433654 | 1438264 | 16.084 | 4682127 | 1767616 |
| 8. | 2-Hydroxypropyl stearate | C21H42O3 | 343.32067 | 1.5 | 17.269 | 38981 | 1214 | 16.254 | 46373 | 1025 |
| 9. | (2S)-1-Hydroxy-3-(tetradecanoyloxy)-2-propanyl (9Z)-9-octadecenoate | C35H66O5 | 567.49830 | 1.3 | 19.231 | 285866 | 10547 | 17.976 | 348859 | 14426 |
| 10. | 3-Phorbinepropanoic acid, 3,4-didehydro-9-ethenyl-14-ethyl-24,25-dihydro-21-(methoxycarbonyl)-4,8,13,18-tetramethyl-20-oxo-, (2E)-3,7,11,15-tetramethyl-2-hexadecen-1-yl ester | C55H72N4O5 | 869.55755 | 1.1 | 20.373 | 4307 | 1110897 | 19.066 | 5350 | 1005380 |
| 11. | Methyl (3R,10Z,14Z,20Z,22S,23S)-12-ethyl-3-hydroxy-13,18,22,27-tetramethyl-5-oxo-23-(3-oxo-3-{[(2E,7R,11R)-3,7,11,15-tetramethyl-2-hexadecen-1-yl]oxy}propyl)-17-vinyl-4-oxa-8,24,25,26-tetraazahexacyclo [19.2.1.16,9.111,14.116,19.02,7]heptacosa-1(24),2(7),6(27),8,10,12,14,16,18,20-decaene-3-carboxylate | C55H74N4O7 | 903.56303 | 1.8 | 20.441 | 45606 | 2940710 | 19.987 | 38274 | 2268701 |
| 12. | 3-Phorbinepropanoic acid, 9-acetyl-14-ethylidene-13,14-dihydro-21-(methoxycarbonyl)-4,8,13,18-tetramethyl-20-oxo-, 3,7,11,15-tetramethyl-2-hexadecen-1-yl ester | C55H74N4O6 | 887.56811 | 1.9 | 20.594 | 332532 | 11277103 | 19.953 | 401031 | 13903240 |
| 13. | Loliolide | C11H16O3 | 197.11722 | 1.2 | 6.225 | 88150 | 4603 | 6.323 | 86765 | 5693 |
| 14. | Isoamijiol oxidation product * | C20H30O2 | 303.23186 | 6.2 | 14.544 | 378362 | 2372 | 14.328 | 352017 | 2346 |
| 15. | (3aR,4aR,6S,8aR)-1-Isopropyl-3a,8a-dimethyl-5-methylene-2,3a,4,5,6,7,8,8a,9,10-decahydrobenzo[f]azulene-4a,6(3H)-diol (Isoamijiol) | C20H32O2 | 305.24751 | 0.5 | 15.974 | 122683 | 17514 | 15.522 | 104432 | 22138 |
| 16. | β-Stigmasterol | C29H46 | 395.36723 | 1.6 | 18.276 | 36308 | 364233 | 17.634 | 33268 | 459395 |
| 17. | (3β,6α)-14-Methylergosta-8,24(28)-diene-3,6-diol | C29H48O2 | 429.37271 | 3.4 | 18.395 | 784665 | 96119 | 17.480 | 980078 | 117567 |
| 18. | Fucoxanthin | C42H58O6 | 659.43062 | 2.1 | 14.873 | 2232648 | 3500 | 15.122 | 2697740 | 4095 |
| 19. | Pheophytin b | C55H72N4O6 | 885.55246 | 8.7 | 20.134 | 39490 | 467361 | 19.765 | 40024 | 391298 |
| 20. | Pheophytin a | C55H74N4O5 | 871.57320 | 2.1 | 20.815 | 5854 | 61538472 | 20.106 | 7418 | 57737924 |
| 21. | Hexadecasphinganine | C16H35NO2 | 274.27410 | 1.0 | 9.507 | 3186185 | 2813931 | 7.789 | 3952304 | 3361364 |
A-USP L7 (Acquity BEH C8) column.; B-USP L11 (Acquity CSH phenyl-hexyl) column; *—exact compound not determined; **—the smallest error for both columns.