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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Oncogene. 2021 Nov 2;41(2):268–279. doi: 10.1038/s41388-021-02089-6

Figure 5. Validation of miR-3662-HBP1 axis-mediated regulation of the Wnt/β-catenin signaling pathway in BT-20 cells.

Figure 5.

A. Expression levels and nuclear localization of β-catenin by IF analysis in scrambled control and KO cells. B. Quantification of expression levels of nuclear/cytoplasmic β-catenin and their ratio. C, D. Western blot and quantitative analyses of expression of HBP1 and Wnt signaling proteins in miR-3662 scrambled control and KO cells. E, F. Expression of HBP1 protein determined by IHC analysis in scrambled control and KO xenograft tumors from NSG mice. G, H. Western blot and quantitative analyses of expression of HBP1 and Wnt signaling proteins in KO, scrambled control, and HBP1 siRNA-transfected KO cells. Data are presented as means ± SD. P values were determined by a two-tailed t-test. Scr, scrambled control; KO, knockout. All experiments were repeated three times.