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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Biomed Pharmacother. 2021 Dec 1;145:112382. doi: 10.1016/j.biopha.2021.112382

Fig. 5.

Fig. 5.

G3BP is absolutely required for recruitment of selected SG markers into CisPt foci. (A) Verification of ΔΔG3BP1/2 mutant and its rescue ΔΔG3BP1/2 + G3BP1 counterpart. All type of cells (parental U2OS, ΔΔG3BP1/2 mutant and ΔΔG3BP1/2 + G3BP1 rescue) were grown till 80% confluency. Then whole protein lysate was isolated and standard western blot against G3BP1 protein was executed. Tubulin β (Tub) was applied as a loading control. (B) Recruitment of CisPt foci in SG-competent (parental U2OS, ΔΔG3BP1/2 + G3BP1) and SG-incompetent (ΔΔG3BP1/2) U2OS cells. Classical marker of SG (Caprin1, HuR, TIAR and TIA-1) were applied. (C) Quantification of CisPt foci in SG-competent (parental U2OS, ΔΔG3BP1/2 + G3BP1) and SG-incompetent (ΔΔG3BP1/2) U2OS cells as shown in Fig. 5B.