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. 2022 Jan 21;17(1):e0262051. doi: 10.1371/journal.pone.0262051

Table 4. qRT-PCR validation of differential expression of a subset of H/S-DE genes.

Gene Gene Symbol Sahiwal Holstein P value
ADAM Metallopeptidase With Thrombospondin Type 1 Motif 20 ADAMTS20 1.3 ± 0.1 2.6 ± 0.4 0.011
Cyclic GMP-AMP Synthase CGAS 2.8 ± 0.2 1.2 ± 0.1 <0.001
HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5 HERC5 3.7 ± 0.6 1.1 ± 0.1 <0.001
Interferon Alpha IFNA 1.7 ± 0.3 2.1 ± 0.4 n.s.
Interferon Alpha Inducible Protein 6 IFI6 11.8 ± 1.1 2.0 ± 0.4 0.001
Interferon Beta 1 IFNB1 2.3 ± 0.4 5.7 ± 1.1 0.006
Interferon Beta 3 IFNB3 5.1 ± 1.4 2.9 ± 0.7 n.s.
Interferon Induced Protein 44 IFI44 50.0 ± 8.4 5.6 ± 1.4 0.002
Interleukin 6 IL6 2.8 ± 0.6 4.5 ± 1.8 n.s.
Interleukin 23 Subunit Alpha IL23A 1.6 ± 0.2 5.8 ± 3.8 n.s.
ISG15 Ubiquitin Like Modifier ISG15 110.8 ± 26.3 5.0 ± 1.8 0.001
MX Dynamin Like GTPase 1 MX1 191.2 ± 14.4 10.9 ± 8.1 0.005
Nectin Cell Adhesion Molecule 3 NECTIN3 5.4 ± 2.0 48.3 ± 13.8 0.018
2’-5’-Oligoadenylate Synthetase 1 OAS1 231.0 ± 23.5 22.0 ± 17.9 0.001
Prune Homolog 2 With BCH Domain PRUNE2 2.0 ± 0.6 335.3 ± 172.9 0.018
Radical S-Adenosyl Methionine Domain Containing 2 RSAD2 153.4 ± 35.1 25.1 ± 19.2 0.013
Sterile Alpha Motif Domain Containing 9 SAMD9 8.5 ± 1.5 1.4 ± 0.1 0.001
Slit Guidance Ligand 2 SLIT2 2.1 ± 0.5 127.4 ± 65.0 0.007
TNF Superfamily Member 10 TNFSF10 12.5 ± 1.6 3.7 ± 0.9 0.009
Wnt Ligand Secretion Mediator WLS 1.2 ± 0.1 6.7 ± 1.7 0.008